Blog

Key Structural Elements of Unsymmetrical Cyanine Dyes for Highly Sensitive Fluorescence Turn-on DNA Probe

Kakishi Uno, Taeko Sasaki, Nagisa Sugimoto, Hideto Ito, Taishi Nishihara, Shinya Hagihara, Tetsuya Higashiyama, Narie Sasaki, Yoshikatsu Sato and Kenichiro Itami

Chem. Asian J. 201712, 233–238. DOI: 10.1002/asia.201601430

Highlighted in chemistry views
Most accessed article in January 2017 (First Place)
Most accessed article in February 2017 (Third Place)
Most accessed article between March 2016 to February 2017 (22nd Place)
Most accessed article between May 2016 to April 2017 (11th place)

Unsymmetrical cyanine dyes such as thiazole orange are useful for detecting nucleic acids with fluorescence because they dramatically enhance the fluorescence upon binding to nucleic acids. In this paper, we synthesized a series of unsymmetrical cyanine dyes and evaluated their fluorescence properties. A systematic structure-property relationship study has uncovered that the dialkylamino group at the 2-position of quinoline in a series of unsymmetrical cyanine dyes plays a critical role in the fluorescence enhancement. Four newly designed unsymmetrical cyanine dyes showed negligible intrinsic fluorescence in a free-state and strong fluorescence upon binding to double-stranded DNA (dsDNA) with quantum yield 0.53-0.90, which is 2-3 times higher than the previous unsymmetrical cyanine dyes. Detailed analysis of fluorescence lifetime revealed that dialkylamino group at the 2-position of quinoline suppressed non-radiative decay in favor of increasing fluorescence quantum yield. Moreover, these newly developed dyes were able to specifically stain the nucleus in fixed HeLa cells with a confocal laser-scanning microscope.

 

Related post

  1. Iridium Catalysis for C-H Bond A…
  2. Annulative π-Extension (APEX): A…
  3. Oxidative Homocoupling Reaction …
  4. Dehydrogenative Synthesis of 2,2…
  5. Topological molecular nanocarbon…
  6. Polycyclic Arene Synthesis by An…
  7. Infinitene: A Helically Twisted …
  8. Strength of carbon nanotubes dep…

最近の記事

Flickr@Itamilab

天池先輩からコーヒースープ伴夫妻からのお歳暮です!潤さん、宮村さん、ありがとうございます!!武藤さん、ビールありがとうございます!平賀大都わーいやなさん、あつしさん、ありがとうございます!!だいぶ前だけど、Stripes look #ootdHalloween lookラインを洗う時ですら格好良く。戸谷先生教育実習!imageけいしゅう、誕生日おめでとう!誕生日は英吉家!!3年生に名古屋ぼろ勝ちアピール中!!アリシア卒業おめでとう女子会!
PAGE TOP